Volume 1, Issue II, Page 49-54
Identification of Fruit Flies (Diptera: Tephritidae) at Species Level: The Molecular Technique is an Effective Tool
Received: 16 April, 2021 || Accepted: 19 May, 2021 || Published: 19 May, 2021
A b s t r a c t
Today's technology enables us to precisely identify any living thing using a single cell. This technology uses molecular probes that bind only to the DNA of targeted organisms, is based on gene amplification that multiplies a million-fold the DNA of interest, and involves comparing specific pieces of DNA between organisms. There are two important advantages of molecular identification over conventional techniques of microscopic examination that means morphological identification. Identification can be made using a very small amount of material and is much more accurate than with previous methods-a species, a population, or even an individual can be identified. So in accurate species identification, no doubt about the molecular method. For fruit fly identification, the molecular method based on PCR-RFLP, DNA barcoding of the COI gene and/or polymerase chain reaction (PCR) with species-specific primers is the commonly used and accurate method of identifying species level.
Keywords: Fruit fly, DNA barcoding, COI gene, economic important and species specific primers.
Copyright information: Copyright © 2021 Author(s) retain the copyright of this article. This work is licensed under a Creative Commons Attribution 4.0 International License
To cite this article: Afroz, S., Islam, M.R. (2021). Identification of Fruit Flies (Diptera: Tephritidae) at Species Level: The Molecular Technique is an Effective Tool. International Journal for Asian Contemporary Research, 1 (2): 49-54.
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