Volume 1, Issue II, Page 49-54

Review Article

Identification of Fruit Flies (Diptera: Tephritidae) at Species Level: The Molecular Technique is an Effective Tool

Sultana Afroz*
Sultana Afroz*

*Department of Agriculture Extension, Ministry of Agriculture, Bangladesh. Email: [email protected]

and Md. Rashedul Islam
Md. Rashedul Islam

Lincoln University Missouri, USA.


Received: 16 April, 2021 || Accepted: 19 May, 2021 || Published: 19 May, 2021

 

A b s t r a c t

Today's technology enables us to precisely identify any living thing using a  single cell. This technology uses molecular probes that bind only to the DNA  of targeted organisms, is based on gene amplification that multiplies a  million-fold the DNA of interest, and involves comparing specific pieces of  DNA between organisms. There are two important advantages of molecular  identification over conventional techniques of microscopic examination that  means morphological identification. Identification can be made using a very  small amount of material and is much more accurate than with previous  methods-a species, a population, or even an individual can be identified. So  in accurate species identification, no doubt about the molecular method. For  fruit fly identification, the molecular method based on PCR-RFLP, DNA  barcoding of the COI gene and/or polymerase chain reaction (PCR) with  species-specific primers is the commonly used and accurate method of  identifying species level.

 

Keywords: Fruit fly, DNA barcoding, COI gene, economic important and  species specific primers.


Copyright information: Copyright © 2021 Author(s) retain the copyright of this article. This work is licensed under a Creative Commons Attribution 4.0 International License


    To cite this article: Afroz, S., Islam, M.R. (2021). Identification of Fruit Flies (Diptera: Tephritidae) at Species Level: The Molecular Technique is an Effective Tool. International Journal for Asian Contemporary Research, 1 (2): 49-54.  


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