H&N Publications INTERNATIONAL

Volume 1, Issue I, Page 31-38

Research Article

Fabrication, Characterization and Antifungal Activity Studies of Silk Fibroin Hydrogel as a Potential Controlled Release of Fluconazole

Tomal Roy
Tomal Roy

Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh.

and Rezaul Haque Ansary*
Rezaul Haque Ansary*

*Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh. Email: [email protected]


Received: 02 April, 2021 || Accepted: 30 April, 2021 || Published: 3 May, 2021

Article info

Received: 02 April, 2021

Accepted: 30 April, 2021

Published: 3 May, 2021

Available in online: 03 May, 2021

 

*Corresponding author:

Email: [email protected]

Abstract

Silk is a natural protein-based fiber derived from the Bombyx mori and certain insects. The research aim is to develop silk fibroin (SF) hydrogels loaded model drug fluconazole to obtain controlled release profile for better patient compliance. An eco-friendly technique was introduced to prepare fibroin hydrogel by treating three different organic solvent of ethanol, propanol, and glycerol by adding 2% (w/v) silk fibroin aqueous solution 37 ºC. The hydrogel having higher SF content showed more stability and slower degradation rate. Then, to prove the potential of SF hydrogel as carriers for drug delivery, fluconazole was absorbed in the hydrogel. All hydrogel released fluconazole in a controllable manner, possibly due to the hydrophobic interaction between fluconazole and crystalline domain of SF. The fibroin hydrogel was characterized by using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Thermo gravimetrical Analysis (TGA), Differential scanning calorimetry (DSC). The encapsulation efficiency and release profile of fluconazole was studied by UV-VIS spectrometry. The surface morphology of the hydrogel was affected by the formulation conditions. Better encapsulation efficiency (85.5±1.67%, 73.4±1.76%, 71.6±1.35%, respectively) of fluconazole for F16, F17, F18 formulation were achieved when glycerol, propanol, ethanol was used in the formulation. The release profile showed an initial burst release for fluconazole then a controlled release for the next 20 hours. The antifungal activity of hydrogel incorporated drug showed a positive response against Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be a good candidate for controlled topical delivery of fluconazole.

Keywords: Silk fibroin, controlled release, hydrogels, drug delivery.

References

  1. Ansary R. H, Roy T., Asraf A., Easmin S. (2020). Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels, American Journal of Nano Research and Applications, 8(2): 28-34
  2. Ansary R. H., Awang M. B., Rahman M. M. (2014). Biodegradable poly (D, L-lactic-co-glycolic acid)-based micro/nanoparticles for sustained release of protein drugs-A review. Tropical Journal of Pharmaceutical Research, 13 (7): 1179-1190.
  3. Ansary R. H., Rahman M. M., Awang M. B., Katas H., Hadi H., Doolaanea A. A. (2016). Preparation, characterization, and in vitro release studies of insulin-loaded double-walled poly (lactide-co-glycolide) microspheres. Drug Delivery and Translational Research, (3): 308-318.
  4. Ansary R. H., Rahman M. M., Awang M. B., Katas H., Hadi H.,Mohamed F., Doolaanea A. A., Kamaruzzaman Y. B. (2016). Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly (lactide-co-glycolide) microspheres. Archives of Pharmacal Research, 39 (9): 1242-1256.
  5. Chen Xin, Cai H., Ling S., Z. Shao, and Y. Hung (2012). Conformation Transition of Bombyx mori Silk Protein Monitored by Time-Dependent Fourier Transform Infrared (FT-IR) Spectroscopy: Effect of Organic Solvent. Applied Spectroscopy, 66:696-699
  6. Fatema U. K., Rahman M. M., Islam M. R., Mollah M. Y., Susan M. A. (2018). Silver/poly (vinyl alcohol) nanocomposite film prepared using water in oil microemulsion for antibacterial applications. Journal of Colloid and Interface Science, 514: 648-655.
  7. Fatema U. K., Rahman M. M., Islam M. R., Mollah M. Y., Susan M. A. (2018). Silver/poly (vinyl alcohol) nanocomposite film prepared using water in oil microemulsion for antibacterial applications. Journal of Colloid and Interface Science, 514: 648-655.
  8. Guerette P.A., Ginzinger D.G., Weber B.H., J.M. Gosline (1996). Silk properties determined by gland-specific expression of a spider fibroin gene family, Science 272 112–115.
  9. Hanawa T., Watanabe A., Tsuchiya T., Ikoma R., Hidaka M., Sugihara M. (1995). New oral dosage form for elderly patients: preparation and characterization of silk fibroin gel. Chemical and Pharmaceutical Bulletin, 43(2): 284-288.
  10. Mariana Agostini de Moraes, Cynthia Regina Albrecht Mahl,Mariana Ferreira Silva,Marisa Masumi Beppu (2014). Formation of silk fibroin hydrogel and evaluation of its drug release Profile, applied polimer science, vol-41802
  11. Matsumoto A., J. Chen, A.L. Collette, U.J. Kim, G.H. Altman, P. Cebe, D.L. Kaplan (2006). Mechanisms of silk fibroin sol–gel transitions, J. Phys. Chem. B 110: 21630–21638.
  12. Ribeiro M., de Moraes M. A., Beppu M. M., Monteiro F. J., Ferraz M. P. (2014). The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels. Biomatterials, 4 (1): 28536.
  13. Safdari M, Shakiba E, Kiaie SH, Fattahi A. (2016). Preparation and characterization of Ceftazidime loaded electrospun silk fibroin/gelatin mat for wound dressing. Fibers and Polymers, 17:744-50.
  14. Sharma G, Van Der Walle C. F, Kumar M. R. (2013). Antacid co-encapsulated polyester nanoparticles for peroral delivery of insulin: Development, pharmacokinetics, biodistribution and pharmacodynamics. International Journal of Pharmaceutics, 440 (1): 99-110.
  15. Su, D., Jiang L, Chen X, Dong J, Shao Z. (2016). Enhancing the Gelation and Bioactivity of Injectable Silk Fibroin Hydrogel with Laponite Nanoplatelets. ACS applied materials & interfaces, 8:9619-28.
  16. Tanaka C., Asakura T. (2009). Synthesis and characterization of cell-adhesive silk-like proteins constructed from the sequences of Anaphe silk fibroin and fibronectin, Biomacromolecules, 10:923–928.
  17. Wang Y., H.-J. Kim, G. Vunjak-Novakovic, D.L. Kaplan (2006). Stem cell-based tissue engineering with silk biomaterials, Biomaterials, 27: 6064–6082.

This work is licensed under a Creative Commons Attribution 4.0 International License.


  Article View: 2944 times

      

Quick Links

Latest Article

First Report on The Occurrence of Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on Ginger (Zingiber officinale) in Bangladesh

Performance of Different Arbuscular Mycorrhizae Species on The Productivity of Rice Under Field Condition

Selecting Parents for Hybridization with Multivariate Analysis Approach to Improve Country Bean (Dolichos lablab L.) Yield

Efficacy of Insecticides in Decreasing the Population of Aphids (Aphis fabae) and Pod Borer (Maruca vitrata) in Country Bean

Genetic Analysis of Fiber Yield Associated Anatomical Traits in Tossa Jute (Corchorus olitorius L.)

Morphological Characterization of the Varietal Traits of Jute and Kenaf Seeds in Bangladesh